collagen type iv alpha 2 chain col4a2  (Proteintech)

Journal: Journal of Pharmaceutical Analysis

Article Title: Signatures of proteomics and glycoproteomics revealed liraglutide ameliorates MASLD by regulating specific metabolic homeostasis in mice

doi: 10.1016/j.jpha.2025.101273

Figure Lengend Snippet: Identification and validation of potential signature molecules affected by liraglutide (Lira). (A) Protein-protein interaction (PPI) analysis of differential proteins and glycoproteins in major pathways. Node size reflects degree value, with red representing glycoproteins and blue representing proteins. Color-coded segments show pathway associations. (B) Messenger RNA (mRNA) expression of Acaa2 , Lamc1 , and Col4a2 (for proteins), and Acox1 , Gclc , and Shmt2 (for glycoproteins). (C) Protein expression of laminin subunit gamma-1 (LAMC1), collagen type IV alpha-2 chain (COL4A2), and acetyl-CoA acyltransferase 2 (ACAA2) (for proteins), and glutamate-cysteine ligase catalytic subunit (GCLC) (for glycoproteins). Data is presented as mean ± standard deviation (SD) ( n = 3 per group). P values were calculated using Student's t -test or one-way analysis of variance (ANOVA) with Tukey's comparisons. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. ns: not significant. PPAR: peroxisome proliferators-activated receptor; ECM: extracellular matrix; ND: normal diet; HFD: high-fat diet; HFDL: HFD + Lira; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.

Article Snippet: The primary antibodies used in our study were as follows: laminin subunit gamma-1 (LAMC1) (1:8000, 67706-1-Ig; Proteintech Group, Inc., Wuhan, China), collagen type IV alpha-2 chain (COL4A2) (1:5000, 55131-1-AP; Proteintech Group, Inc.), acetyl-CoA acyltransferase 2 (ACAA2) (1:5000, 11111-1-AP; Proteintech Group, Inc.), glutamate-cysteine ligase catalytic subunit (GCLC) (1:6000, 12601-1-AP; Proteintech Group, Inc.), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:100000, 60004-1-Ig; Proteintech Group, Inc.), and α-tubulin (1:6000, PTM-5001; PTM BIO, Hangzhou, China).

Techniques: Biomarker Discovery, Expressing, Standard Deviation

Journal: Frontiers in Genetics

Article Title: Identification of effective diagnostic biomarker and immune cell infiltration characteristics in acute liver failure by integrating bioinformatics analysis and machine-learning strategies

doi: 10.3389/fgene.2022.1004912

Figure Lengend Snippet: Top 30 hub genes screened by MCC and Degree methods.

Article Snippet: The membrane was then blocked with 5% nonfat dried milk in Tris-buffered saline containing Tween-20 for 1 h. After that, the membrane was incubated overnight with primary antibodies at 4°C: collagen type IV alpha 2 chain (COL4A2) (A7657, 1:1000, ABclonal), GAPDH (ab9485, 1:1000, Abcam).

Techniques:

Journal: Frontiers in Genetics

Article Title: Identification of effective diagnostic biomarker and immune cell infiltration characteristics in acute liver failure by integrating bioinformatics analysis and machine-learning strategies

doi: 10.3389/fgene.2022.1004912

Figure Lengend Snippet: Identification of diagnostic biomarker of ALF via the comprehensive strategy. (A) LASSO logistic regression algorithm to screen diagnostic markers. (B) SVM-RFE algorithm to screen biomarkers. (C) Venn diagram to identify intersected DIAGNOSTIC biomarkers screened by the LASSO and SVM-RFE. (D) The expression level of COL4A2 in the validation set. (E) The protein levels of COL4A2 in normal and ALF mice models. The ROC curve of the gene signature in the testing set (F) and validation set (G) .

Article Snippet: The membrane was then blocked with 5% nonfat dried milk in Tris-buffered saline containing Tween-20 for 1 h. After that, the membrane was incubated overnight with primary antibodies at 4°C: collagen type IV alpha 2 chain (COL4A2) (A7657, 1:1000, ABclonal), GAPDH (ab9485, 1:1000, Abcam).

Techniques: Diagnostic Assay, Biomarker Discovery, Expressing

Journal: Frontiers in Genetics

Article Title: Identification of effective diagnostic biomarker and immune cell infiltration characteristics in acute liver failure by integrating bioinformatics analysis and machine-learning strategies

doi: 10.3389/fgene.2022.1004912

Figure Lengend Snippet: Pearson correlation of immune infiltrating cells with the signature genes. (A) Correlation between COL4A2 and infiltrating immune cells. (B) Plasma cells. (C) Activated CD4 memory T cells. (D) Monocytes. (E) CD8 T cells. (F) Follicular T helper cells. (G) Activated Mast cells. (H) Resting CD4 memory T cells.

Article Snippet: The membrane was then blocked with 5% nonfat dried milk in Tris-buffered saline containing Tween-20 for 1 h. After that, the membrane was incubated overnight with primary antibodies at 4°C: collagen type IV alpha 2 chain (COL4A2) (A7657, 1:1000, ABclonal), GAPDH (ab9485, 1:1000, Abcam).

Techniques: Clinical Proteomics

Journal: Frontiers in Genetics

Article Title: Identification of effective diagnostic biomarker and immune cell infiltration characteristics in acute liver failure by integrating bioinformatics analysis and machine-learning strategies

doi: 10.3389/fgene.2022.1004912

Figure Lengend Snippet: Construction of the COL4A2–miRNA Regulatory Network (A) Venn diagram of the intersection of targeted miRNAs screened by miRWalk, miRDB and TargetScan databases. (B) COL4A2–miRNA regulatory network. The square represents gene, and the circle represents miRNA. The green frame represents down-regulated miRNA, and pink frame means up-regulated gene.

Article Snippet: The membrane was then blocked with 5% nonfat dried milk in Tris-buffered saline containing Tween-20 for 1 h. After that, the membrane was incubated overnight with primary antibodies at 4°C: collagen type IV alpha 2 chain (COL4A2) (A7657, 1:1000, ABclonal), GAPDH (ab9485, 1:1000, Abcam).

Techniques: